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Title HIV protocols [electronic resource] / edited by Nelson L. Michael and Jerome H. Kim.

Published Totowa, N.J. ; Humana Press, c1999.


Location Call No. Status
Physical description 1 online resource (xiii, 421 p.) : ill.
Series Methods in molecular medicine ; 17
Methods in molecular medicine ; 17.
Bibliography Includes bibliographical references and index.
Contents Isolation and expansion of hiv from cells and body fluids by coculture / J.R. Lane-Quantitative hiv culture / J.R. Lane-Quantitation of cell-free hiv by reverse transcriptase activity / J.R. Lane-Determination of syncytium-inducing phenotype of primary hiv-1 isolates using mt-2 cells / M.K. Louder and J.R. Mascola-Determination of hiv-1 chemokine coreceptor tropism using transduced human osteosarcoma (hos) cells / M.K. Louder and J.R. Mascola-Use of luciferase reporter viruses for studying hiv entry / R. Liu, B. Chen and N.R. Landau-A cell-cell fusion assay to monitor hiv-1 env interactions with chemokine receptors / A.L. Edinger and R.W. Doms-Methods of culturing hiv-1 from semen / B.L. Gilliam-Detection of hiv-1 nucleic acids by southern blotting / R.A. McDonald-Detection of hiv-1 nucleic acids by northern blotting / R.A. McDonald and C.A. Smith-Rapid identification of cloned hiv-1 fragments / W. Dong, A.K. Roy and Y. Li-Pcr amplification and cloning of virtually full-length hiv-1 provirus / M. Salminen-Quantitation of hiv-1gag DNA and rna from single frozen cell pellets / M. Vahey, S.C. Barrick and M. Nau-Quantitative pcr for hiv-1 proviral DNA / D. Zella and P. Secchiero-Diagnosis and direct automated sequencing of hiv-1 from dried blood spots (dbs) collected on filter paper / S.A. Cassol ... [et al.]-Quantification of hiv-1 rna in dried plasma spots (dps) : A field approach to therapeutic monitoring / S.A. Cassol ... [et al.]-Quantitation of hiv-1 rna in genital secretions / B.L. Gilliam-Detection of nucleic acids in cells and tissues by in situ polymerase chain reaction / O. Bagasra ... [et al.]-Determination of the promoter activity of hiv-1 using the chloramphenicol acetyltransferase reporter gene assay / L. Tse and N.L. Michael-In vitro techniques for studies of hiv-1 promoter activity / F. Romerio and D.M. Margolis-Detection of polymorphisms in the hiv-1 coreceptor ccr5 using single-strand conformation polymorphism / M.P. Martin and M. Carrington-Sequence-specific priming as a rapid screen for known mutations / M. Carrington-Quantitation of hiv-1 entry cofactor expression / J.L. Riley and R.G. Carroll-Retrovirus-mediated gene transduction of supt1 cells / J.T. Lin and J.H. Kim-Cloning of hiv single-strand DNA binding protein from human lymphocyte lambda gt11 expression library with [(32)p]-oligomers / S.J. Hur and D. Lai-Telomeric terminal restriction fragment (trf) / S. Kaushal-Serologic analysis by enzyme-linked immunosorbent assay (elisa) / M. Krider and L.D. Loomis-Price-Quantitative immunoblotting with fusion proteins / M.M. Mitchell and L.D. Loomis-Price-Analysis of antibody interactions with hiv-1 envelope expressed on the surface of acutely infected h9 cells / T.C. Vancott and P.L. Hallberg-Analysis of antibody-antigen interactions using surface plasmon resonance / T.C. Vancott-Measurement of hiv-1 specific and total antibody secreting cells by elispot / F.W. van Ginkel ... [et al.]-Linear epitope mapping by the pepscan method / L.D. Loomis-Price-Neutralization of hiv-1 infection of human peripheral blood mononuclear cells (pbmc) : Antibody dilution method / J.R. Mascola-Neutralization of hiv-1 infection of human peripheral blood mononuclear cells (pbmc) : Infectivity reduction method / J.R. Mascola-Collection and processing of mucosal secretions from hiv-1 infected women / A.W. Artenstein and T.C. Vancott-Collection and processing of mucosal secretions from mice / R.W. Kaminski and T.C. Vancott-Lymphocyte proliferation assay / K.V. Sitz and D.L. Birx-Hiv-1-specific cytotoxic t-cell assays / J.H. Cox-Hiv-1-specific antibody-dependent cellular cytotoxicity (adcc) / J.H. Cox-Evaluation of natural killer cell activity / J.H. Cox-Generation and expansion of hiv-1 cd4+ antigen-specific t cell lines / S. Ratto-Kim-Immunophenotyping and assessment of cell function by three-color flow cytometry of peripheral blood lymphocytes / S.P. Perfetto and G. McCrary-Detection of apoptosis in hiv-infected ceil populations using tunel / P.J. Blair and S.P. Perfetto.
Notes Description based on print version record.
Other author Michael, Nelson.
Kim, Jerome H.
Subject AIDS (Disease) -- Laboratory manuals.
HIV (Viruses) -- Laboratory manuals.
HIV infections -- Laboratory manuals.
ISBN 9781592596010
9780896033696 (alk. paper)
0896033694 (alk. paper)