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Author Price, Nicholas C.

Title Fundamentals of enzymology : the cell and molecular biology of catalytic proteins / Nicholas C. Price and Lewis Stevens.

Published Oxford ; New York : Oxford University Press, 1999.

Copies

Location Call No. Status
 UniM BioMed  572.7 PRIC    AVAILABLE
Edition 3rd ed.
Physical description pages cm
Bibliography Includes bibliographical references and index.
Contents 1.2 Historical aspects 1 -- 1.3 Remarkable properties of enzymes as catalysts 4 -- 1.4 Cofactors 7 -- 1.5 Nomenclature and classification of enzymes 8 -- Chapter 2 Purification of enzymes 15 -- 2.2 Why isolate enzymes? 15 -- 2.3 Objectives and strategy in enzyme purification 16 -- 2.4 Choice of source 17 -- 2.5 Methods of homogenization 20 -- 2.6 Methods of separation 21 -- 2.7 How to judge the success of a purification procedure 34 -- 2.8 Examples of purification procedures 36 -- 2.9 Conclusions from the examples of enzyme purification 43 -- Chapter 3 Structure of enzymes 47 -- 3.2 Determination of M[subscript r] 48 -- 3.3 Determination of amino-acid composition and primary structure 54 -- 3.4 Determination of secondary and tertiary structure 75 -- 3.5 Determination of quaternary structure 100 -- 3.6 Unfolding and folding of enzymes 106 -- Chapter 4 An introduction to enzyme kinetics 118 -- 4.1 Outline of the chapter 118 -- 4.2 How do we obtain kinetic data? 119 -- 4.3 How do we analyse kinetic data? 121 -- 4.4 Pre-steady-state kinetics 144 -- Chapter 5 Mechanism of enzyme action 154 -- 5.2 Definition of the mechanism of an enzyme-catalysed reaction 154 -- 5.3 Background to catalysis 155 -- 5.4 Experimental approaches to the determination of enzyme mechanisms 162 -- 5.5 Examples of enzyme mechanisms 177 -- 5.6 Concluding comments on enzyme mechanisms 212 -- Chapter 6 Control of enzyme activity 217 -- 6.2 Control of the activities of single enzymes 218 -- 6.3 Control of metabolic pathways 237 -- 6.4 Examples of control of metabolic pathways 249 -- Chapter 7 Enzymes in organized systems 273 -- 7.2 Organized enzyme systems 273 -- 7.3 RNA polymerase from E. coli 274 -- 7.4 Occurrence and isolation of multienzyme proteins 281 -- 7.5 Phylogenetic distribution of multienzyme proteins 282 -- 7.6 Properties of multienzyme proteins 283 -- 7.7 Pyruvate dehydrogenase multienzyme complex and related systems 285 -- 7.8 Glycine decarboxylase multienzyme complex 294 -- 7.9 Tryptophan synthase multienzyme complex from E. coli 294 -- 7.10 Carbamoyl phosphate synthase and the associated enzymes of the pyrimidine and arginine biosynthetic pathways in E. coli, fungi, and mammalian cells 300 -- 7.11 Multienzyme polypeptides: fatty-acid synthase and the arom complex (AROM enzyme) 304 -- 7.12 Enzymes involved in DNA synthesis 315 -- 7.13 Glycogen particle 315 -- Chapter 8 Enzymes in the cell 321 -- 8.2 Intracellular compartmentation 322 -- 8.3 Compartmentation of metabolic pathways 333 -- 8.4 Vectorial organization of enzymes associated with membranes 347 -- 8.5 Concentrations of enzymes and substrates in vivo 355 -- Chapter 9 Enzyme turnover 370 -- 9.2 Kinetics of enzyme turnover 371 -- 9.3 Methods for measurement of rates of enzyme turnover 373 -- 9.4 Results from measurements of rates of enzyme turnover 375 -- 9.5 Possible correlations between the rates of turnover and the structure and function of enzymes 376 -- 9.6 Mechanisms of protein degradation 377 -- 9.7 Significance of enzyme turnover 389 -- 9.8 Other processes in which intracellular proteolysis is important 394 -- Chapter 10 Clinical aspects of enzymology 400 -- 10.2 Determination of enzyme activities for clinical diagnosis 401 -- 10.3 Clinical enzymology of liver disease 404 -- 10.4 Clinical enzymology of heart disease 406 -- 10.5 Other enzyme activities that become elevated in serum in disease 408 -- 10.6 Detection and significance of enzyme deficiencies 411 -- 10.7 Enzyme inhibitors and drug design 415 -- 10.8 Use of enzymes to determine the concentrations of metabolites of clinical importance 416 -- 10.9 Enzyme therapy 424 -- Chapter 11 Enzyme technology 430 -- 11.2 Use of microorganisms in brewing and cheesemaking 430 -- 11.3 Use of microorganisms in the production of organic chemicals 433 -- 11.4 Use of isolated enzymes in industrial processes 433 -- 11.5 Immobilized enzymes 440.
Summary Since the publication of the successful and popular second edition of Fundamentals of Enzymology in 1989, a large increase in the knowledge of several aspects of enzymology has occurred -- particularly in the areas of structural characterization of enzymes and bioinformatics. This new edition places appropriate emphasis on the new knowledge while consolidating the strengths of the previous editions. As before, this volume gives a thorough overview of the field that includes: enzyme purification and characterization; enzyme structure (including information on World Wide Web sites); enzyme kinetics; the mechanisms and control of enzyme action; enzyme folding; how enzymes act in vivo; enzyme synthesis and degradation; and clinical and industrial applications of enzymology. Furthermore, the integration of these themes is stressed throughout the book, making it useful for advanced undergraduates in biochemistry and molecular biology.
Other author Stevens, Lewis.
Subject Enzymes.
ISBN 019850229X (paperback: alk. paper)
0198502303 (hb : alk. paper)