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PRINTED BOOKS
Author Brown, T. A. (Terence A.)

Title Gene cloning and DNA analysis : an introduction / T.A. Brown.

Published Oxford ; Hoboken, N.J. : Wiley-Blackwell, 2010.

Copies

Location Call No. Status
 UniM BioMed  572.8633 BROW    AVAILABLE
 UniM Vet W  572.8633 BROW    AVAILABLE
Edition 6th ed.
Physical description xvi, 320 pages : colour illustrations, colour maps ; 26 cm
Notes Previous ed: 2006.
Bibliography Includes bibliographical references and index.
Contents Machine derived contents note: Preface to the Sixth Edition. -- Part 1: The Basic Principles of Gene Cloning and DNA Analysis. -- 1 Why Gene Cloning and DNA Analysis are Important. -- 1.1 The early development of genetics. -- 1.2 The advent of gene cloning and the polymerase chain reaction. -- 1.3 What is gene cloning? -- 1.4 What is PCR? -- 1.5 Why gene cloning and PCR are so important. -- 1.6 How to find your way through this book. -- 2 Vectors for Gene Cloning: Plasmids and Bacteriophages. -- 2.1 Plasmids. -- 2.2 Bacteriophages. -- 3 Purification of DNA from Living Cells. -- 3.1 Preparation of total cell DNA. -- 3.2 Preparation of plasmid DNA. -- 3.3 Preparation of bacteriophage DNA. -- 4 Manipulation of Purified DNA. -- 4.1 The range of DNA manipulative enzymes. -- 4.2 Enzymes for cutting DNA ? restriction endonucleases. -- 4.3 Ligation ? joining DNA molecules together. -- 5 Introduction of DNA into Living Cells. -- 5.1 Transformation ? the uptake of DNA by bacterial cells. -- 5.2 Identification of recombinants. -- 5.3 Introduction of phage DNA into bacterial cells. -- 5.4 Identification of recombinant phages. -- 5.5 Introduction of DNA into non-bacterial cells. -- 6 Cloning Vectors for E. coli. -- 6.1 Cloning vectors based on E. coli plasmids. -- 6.2 Cloning vectors based on M13 bacteriophage. -- 6.3 Cloning vectors based on l bacteriophage. -- 6.4 ? and other high capacity vectors enable genomic libraries to be constructed. -- 6.5 Vectors for other bacteria. -- 7 Cloning Vectors for Eukaryotes. -- 7.1 Vectors for yeast and other fungi. -- 7.2 Cloning vectors for higher plants. -- 7.3 Cloning vectors for animals. -- 8 How to Obtain a Clone of a Specific Gene. -- 8.1 The problem of selection. -- 8.2 Direct selection. -- 8.3 Identification of a clone from a gene library. -- 8.4 Methods for clone identification. -- 9 The Polymerase Chain Reaction. -- 9.1 The polymerase chain reaction in outline. -- 9.2 PCR in more detail. -- 9.3 After the PCR: studying PCR products. -- 9.4 Real time PCR enables the amount of starting material to be quantified. -- Part 2: The Applications of Gene Cloning and DNA Analysis in Research. -- 10 Sequencing Genes and Genomes. -- 10.1 The methodology for DNA sequencing. -- 10.2 How to sequence a genome. -- 11 Studying Gene Expression and Function. -- 11.1 Studying the RNA transcript of a gene. -- 11.2 Studying the regulation of gene expression. -- 11.3 Identifying and studying the translation product of a cloned gene. -- 12 Studying Genomes. -- 12.1 Genome annotation. -- 12.2 Studies of the transcriptome and proteome. -- Part 3:The Applications of Gene Cloning and DNA Analysis in Biotechnology. -- 13 Production of Protein from Cloned Genes. -- 13.1 Special vectors for expression of foreign genes in E. coli. -- 13.2 General problems with the production of recombinant protein in E. coli. -- 13.3 Production of recombinant protein by eukaryotic cells. -- 14 Gene Cloning and DNA Analysis in Medicine. -- 14.1 Production of recombinant pharmaceuticals. -- 14.2 Identification of genes responsible for human diseases. -- 14.3 Gene therapy. -- 15 Gene Cloning and DNA Analysis in Agriculture. -- 15.1 The gene addition approach to plant genetic engineering. -- 15.2 Gene subtraction. -- 15.3 Problems with genetically modified plants. -- 16 Gene Cloning and DNA Analysis in Forensic Science and Archaeology. -- 16.1 DNA analysis in the identification of crime suspects. -- 16.2 Studying kinship by DNA profiling. -- 16.3 Sex identification by DNA analysis. -- 16.4 Archaeogenetics ? using DNA to study human prehistory. -- Glossary. -- Index.
Supplements Has companion website.
Subject Molecular cloning.
Nucleotide sequence.
DNA -- Analysis.
Variant Title Gene cloning & DNA analysis : an introduction.
ISBN 9781405181730
1405181737
9781444334074 (hbk.)
1444334077 (hbk.)