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Title Unnatural amino acids [electronic resource] : methods and protocols / edited by Loredano Pollegioni, Stefano Servi.

Published [Totowa, NJ] : Humana Press ; New York : Springer Science+Business Media, LLC, c2012.


Location Call No. Status
Physical description 1 online resource (xiv, 409 p.) : ill.
Series Methods in molecular biology, 1064-3745 ; 794
Methods in molecular biology (Clifton, N.J.) ; v. 794. 1064-3745
Bibliography Includes bibliographical references and index.
Contents Preparation of unnatural amino acids with ammonia-lyases and 2,3-aminomutases -- Multistep enzyme catalyzed reactions for unnatural amino acids -- Enzymatic production of enantiopure amino acids from mono-substituted hydantoin substrates -- Preparation of glutamate analogues by enzymatic transamination -- Carbon-carbon bond-forming enzymes for the synthesis of non-natural amino acids -- Engineering cyclic amidases for non-natural amino acid synthesis -- NMR analysis of unnatural amino acids in natural antibiotics -- Site-specific incorporation of unnatural amino acids as probes for protein conformational changes -- Application of unnatural amino acids to the de novo design of selective antibiotic peptides -- Use of unnatural amino acids to probe structure-activity relationships and mode-of-action of antimicrobial peptides -- Experimental methods for scanning unnatural amino acid mutagenesis -- Genetic incorporation of unnatural amino acids into proteins in yeast -- Site-specific incorporation of unnatural amino acids into proteins in mammalian cells -- Incorporation of unnatural non-alpha-amino acids into the N-terminus of proteins in a cell-free translation system -- Site-specific modification of proteins by the staudinger-phosphite reaction -- Hplc methods for determination of D-aspartate and N-methyl-D-aspartate -- Estimation of chronological age from the racemization rate of L- and D-aspartic acid: How to completely separate enantiomers from dentin -- Enzymatic detection of D-amino acids -- An enzymatic-HPLC assay to monitor endogenous D-serine release from neuronal cultures -- Electrophysiological analysis of the modulation of NMDA-receptors function by D-serine and glycine in the central nervous system -- Biosensors for d-amino acid detection -- Analysis of D-[beta]-aspartyl isomers at specific sites in proteins -- Nutritional value of D-amino acids, d-peptides, and amino acid derivatives in mice -- Preparation and assay of recombinant serine racemase -- Assay of amino acid racemases -- Assays of D-amino acid oxidases -- Enzymes acting on D-amino acid containing peptides.
Other author Pollegioni, Loredano.
Servi, Stefano, 1943-
Subject Amino acids.
Life sciences
Biochemistry, general
Electronic books.
ISBN 9781617793318 (electronic bk.)
1617793310 (electronic bk.)