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Title Hematopoietic stem cell protocols [electronic resource] / edited by Christopher A. Klug and Craig T. Jordan.

Published Totowa, N.J. : Humana Press, c2002.


Location Call No. Status
Physical description 1 online resource (xi, 332 p.) : ill.
Series Methods in molecular medicine ; 63
Methods in molecular medicine ; 63.
Bibliography Includes bibliographical references and index.
Contents Isolation and analysis of hematopoietic stem cells from mouse embryos / E. Dzierzak -- The purification of mouse hematopoietic stem cells at sequential stages of maturation / S.J. Morrison -- Flow cytometry and immunoselection of human stem cells / T.E. Thomas -- Method for purification of human hematopoietic stem cells by flow cytometry / M.J. Reitsma -- Noninvasive measurement of hematopoietic stem cell cycling and turnover by in vivo bromodeoxyuridine incorporation / G.G. Bradford -- Isolation and characterization of primitive hematopoietic cells based on their position in the cell cycle / E.F. Srour -- Hematopoietic colony-forming cells / M. Ogawa -- Long-term culture-initiating cell assays for human and murine cells / C.L. Miller -- The cobblestone-area-forming cell assay / G. Haan -- CFU-S: an assay for pluripotent myelopoietic stem cells / E.A. McCulloch -- Intrathymic injection for analysis of T-cell progenitor activity / L. Jerabek -- Quantitation of murine and human hematopoietic stem cells by limiting dilution analysis in competitively repopulated hosts / S.J. Szilvassy -- Ex vivo expansion of human and murine hematopoietic stem cells / C.L. Miller -- Hematopoietic development of ES cells in culture / G.M. Keller -- Genetic modification of murine hematopoietic stem cells by retroviruses / C.P. Kalberer -- Retroviral transduction of FACS-purified hematopoietic stem cells / C.V. Cotta -- Retroviral-mediated transduction and clonal integration analysis of human hematopoietic stem and progenitor cells / M.A. Dao -- Production of lentiviral vector supernatants and transduction of cellular targets / R.E. Sutton -- Reverse transcriptase-PCR analysis of gene expressiong in hematopoietic stem cells / D. Orlic -- Identification of differentially expressed genes in sorted cell populations by two-dimensional gene-expression fingerprinting / A.V. Belyavsky.
Restrictions Access restricted to authorized subscribers.
Other formats Also issued in print.
Notes Description based on print version record.
Other author Klug, Christopher A.
Jordan, Craig T.
Subject Hematopoietic stem cells -- Laboratory manuals.
SCIENCE -- Life Sciences -- Human Anatomy & Physiology.
MEDICAL -- Anatomy.
Wetenschappelijke technieken.
Electronic books.
ISBN 0896038122 (alk. paper)
9780896038127 (alk. paper)