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Author K诨ler, Thomas.

Title Quantitation of mRNA by Polymerase Chain Reaction [electronic resource] : Nonradioactive PCR Methods / by Thomas K诨ler, Dirk LaǮer, Harald Remke, Anne-Katrin Rost, Barbara Thamm, Barbara Pustowoit.

Published Berlin, Heidelberg : Springer Berlin Heidelberg, 1996.


Location Call No. Status
Physical description 1 online resource (XX, 166 pages 15 illustrations).
Series Springer Labor Manuals
Springer Labor Manuals.
Summary In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques for quantifying nucleic acids in biological and clincial samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quanatitative PCR protocols into their own clinical or biomedical research.
Other author LaǮer, Dirk.
Remke, Harald.
Rost, Anne-Katrin.
Thamm, Barbara.
Pustowoit, Barbara.
Subject Life sciences.
Human genetics.
Analytical biochemistry.
Analytical biochemistry.
Human genetics.
Life sciences.
Electronic books.
ISBN 9783642797125 (electronic bk.)
3642797121 (electronic bk.)