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Title High-throughput next generation sequencing : methods and applications / edited by Young Min Kwon, Steven C. Ricke.

Published New York : Springer, [2011]


Location Call No. Status
 UniM BioMed ST  574.88078 METH  v.733    AVAILABLE
Physical description xi, 308 pages : illustrations (some colour) ; cm.
Series Methods in molecular biology, 1064-3745 ; 733.
Springer protocols.
Methods in molecular biology (Clifton, N.J.) ; v. 733.
Springer protocols (Series)
Contents Helicos single-molecule sequencing of bacterial genomes / Kathleen E. Steinmann ... [et al.] -- Whole-genome sequencing of unculturable bacterium using whole-genome amplification / Yuichi Hongoh and Atsushi Toyoda -- RNA sequencing and quantitation using the helicos genetic analysis system / Tal Raz ... [et al.] -- Transcriptome profiling using single-molecule direct RNA sequencing / Fatih Ozsolak and Patrice M. Milos -- Discovery of bacterial sRNAs by high-throughput sequencing / Jane M. Liu and Andrew Camilli -- Identification of virus encoding microRNAs using 454 FLX sequencing platform / Byung-Whi Kong -- Ribosomal RNA depletion for massively parallel bacterial RNA-sequencing applications / Zhoutao Chen and Xiaoping Duan -- Integrating high-throughput pyrosequencing and quantitative real-time PCR to analyze complex microbial communities / Husen Zhang ... [et al.] -- Tag-encoded FLX amplicon pyrosequencing for the elucidation of microbial and functional gene diversity in any environment / Yan Sun, Randall D. Wolcott, and Scot E. Dowd -- Pyrosequencing of chaperonin-60 (cpn60) amplicons as a means of determining microbial community composition / John Schellenberg ... [et al.] -- Prescreening of microbial populations for the assessment of sequencing potential / Irene B. Hanning and Steven C. Ricke -- Metagenomics / Jack A. Gilbert ... [et al.] -- Metagenomic analysis of intestinal microbiomes in chickens / Taejoong Kim and Egbert Mundt -- Gene expression profiling : metatranscriptomics / Jack A. Gilbert and Margaret Hughes -- High-throughput insertion tracking by deep sequencing for the analysis of bacterial pathogens / Sandy M.S. Wong ... [et al.] -- Determining DNA methylation profiles using sequencing / Suhua Feng ... [ et al.] -- Preparation of next-generation sequencing libraries using Nextera technology : simultaneous DNA fragmentation and adaptor tagging by in vitro transposition / Nicholas Caruccio -- Amplification-free library preparation for paired-end illumina sequencing / Iwanka Kozarewa and Daniel J. Turner -- Target-enrichment through amplification of hairpin-ligated universal targets for next-generation sequencing analysis / Pallavi Singh, Rajesh Nayak, and Young Min Kwon -- 96-plex molecular barcoding for the illumina genome analyzer / Iwanka Kozarewa and Daniel J. Turner.
Bibliography Includes bibliographical references and index.
Other author Kwon, Young Min.
Ricke, Steven C., 1957-
Subject Genomes -- Analysis.
Gene expression -- Analysis.
Gene mapping -- Laboratory manuals.
ISBN 1617790885
9781617790898 (e-isbn)
1617790893 (e-isbn)