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Title Stable isotope labeling by amino acids in cell culture (SILAC) : methods and protocols / edited by Bettina Warscheid.

Published New York, NY : Humana Press, 2014.


Location Call No. Status
Physical description 1 online resource (xiv, 372 pages) : illustrations (some color).
Series Methods in Molecular Biology, 1064-3745 ; 1188
Methods in molecular biology (Clifton, N.J.) ; 1188. 1064-3745
Notes Includes index.
Contents Fifteen years of stable isotope labeling by amino acids in cell culture (SILAC) / Matthias Mann -- Stable isotope labeling by amino acids applied to bacterial cell culture / Boumediene Soufi and Boris Macek -- SILAC labeling of yeast for the study of membrane protein complexes / Silke Oeljeklaus ... [et al.] -- Whole proteome analysis of the protozoan parasite Trypanosoma brucei using stable isotope labeling by amino acids in cell culture and mass spectrometry / Olivera Cirovic and Torsten Ochsenreiter -- Stable isotope labeling by amino acids in cultured primary neurons / Guoan Zhang, Katrin Deinhardt, and Thomas A. Neubert -- SILAC and alternatives in studying cellular proteomes of plants / Annemarie Matthes, Karin Köhl, and Waltraud X. Schulze -- In vivo stable isotope labeling by amino acids in Drosophila melanogaster / Matthias D. Sury, Jia-Xuan Chen, and Matthias Selbach -- Stable isotope labeling for proteomic analysis of tissues in mouse / Soraya Hölper, Aaron Ruhs, and Marcus Krüger -- Identification of novel protein functions and signaling mechanisms by genetics and quantitative phosphoproteomics in Caenorhabditis elegans / Julius Fredens, Kasper ... [et al.] -- SILAC-based temporal phosphoproteomics / Chiara Francavilla ... [et al.] -- Global ubiquitination analysis by SILAC in mammalian cells / Zhiping Wu ... [et al.] -- Quantifying in vivo, site-specific changes in protein Mmthylation with SILAC / Ho-Tak Lau, Karen A. Lewis, and Shao-En Ong -- Applying SILAC for the differential analysis of protein complexes / Karsten Boldt ... [et al.]-- Defining dynamic protein interactions using SILAC-based quantitative mass spectrometry / Xiaorong Wang and Lan Huang -- Identifying nuclear protein-protein interactions using GFP affinity purification and SILAC-based quantitative mass spectrometry / H. Irem Baymaz, Cornelia G. Spruijt, and Michiel Vermeulen -- Analyzing the protein assembly and dynamics of the human spliceosome with SILAC / Carla Schmidt ... [et al.] -- Identification and validation of protein-protein interactions by combining co-immunoprecipitation, antigen competition, and stable isotope labeling / Frederik Sommer ... [et al.] -- Protein correlation profiling-SILAC to study protein-protein interactions / Anders R. Kristensen and Leonard J. Foster -- Autophagosomal proteome analysis by protein correlation profiling-SILAC / Andrea C. Becker and Jörn Dengjel -- Design and application of super-SILAC for proteome quantification / Yair Pozniak and Tamar Geiger -- Proteomics meets genetics : SILAC labeling of Drosophila melanogaster larvae and cells for in vivo functional studies / Alessandro Cuomo ... [et al.] -- Analysis of secreted proteins using SILAC / Jeanette Henningsen, Blagoy Blagoev, and Irina Kratchmarova -- Identification of MicroRNA targets by pulsed SILAC / Markus Kaller ... [et al.] -- MaxQuant for in-depth analysis of large SILAC datasets / Stefka Tyanova, Matthias Mann, and Jürgen Cox.
Notes Description based on online resource; title from PDF title page (SpringerLink, viewed August 6, 2014).
Other author Warscheid, Bettina, editor.
Subject Cell culture -- Laboratory manuals.
Amino acids -- Laboratory manuals.
Stable isotopes -- Laboratory manuals.
Electronic books.
ISBN 9781493911424 (electronic bk.)
1493911422 (electronic bk.)
Standard Number 10.1007/978-1-4939-1142-4