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Cover Art
PRINTED BOOKS
Author Prasad, Paras N.

Title Introduction to biophotonics / Paras Prasad.

Published Hoboken, NJ : Wiley-Interscience, 2003.

Copies

Location Call No. Status
 UniM ERC  571.455 PRAS    AVAILABLE
Physical description xvii, 593 pages : illustrations, ; 25 cm
Contents 1.1 Biophotonics--A New Frontier 1 -- 1.2 An Invitation to Multidisciplinary Education, Training, and Research 2 -- 1.3 Opportunities for Both Basic Research and Biotechnology Development 4 -- 2. Fundamentals of Light and Matter 11 -- 2.1 Nature of Light 12 -- 2.2 Quantized States of Matter 21 -- 2.3 Intermolecular Effects 41 -- 2.4 Three-Dimensional Structures and Stereoisomers 43 -- 3. Basics of Biology 50 -- 3.2 Cellular Structure 52 -- 3.3 Various Types of Cells 58 -- 3.4 Chemical Building Blocks 60 -- 3.5 Interactions Determining Three-Dimensional Structures of Biopolymers 68 -- 3.6 Other Important Cellular Components 72 -- 3.7 Cellular Processes 73 -- 3.8 Protein Classification and Function 82 -- 3.9 Organization of Cells into Tissues 85 -- 3.10 Types of Tissues and Their Functions 87 -- 3.11 Tumors and Cancers 88 -- 4. Fundamentals of Light-Matter Interactions 92 -- 4.1 Interactions Between Light and a Molecule 93 -- 4.2 Interaction of Light with a Bulk Matter 97 -- 4.3 Fate of Excited State 99 -- 4.4 Various Types of Spectroscopy 102 -- 4.5 Electronic Absorption Spectroscopy 105 -- 4.6 Electronic Luminescence Spectroscopy 109 -- 4.7 Vibrational Spectroscopy 113 -- 4.8 Spectroscopy Utilizing Optical Activity of Chiral Media 117 -- 4.9 Fluorescence Correlation Spectroscopy (FCS) 122 -- 5. Principles of Lasers, Current Laser Technology, and Nonlinear Optics 129 -- 5.2 Current Laser Technologies 139 -- 5.3 Quantitative Description of Light: Radiometry 142 -- 5.4 Nonlinear Optical Processes with Intense Laser Beam 143 -- 5.5 Time-Resolved Studies 152 -- 5.6 Laser Safety 154 -- 6. Photobiology 159 -- 6.1 Photobiology--At the Core of Biophotonics 160 -- 6.2 Interaction of Light with Cells 160 -- 6.3 Interaction of Light with Tissues 168 -- 6.4 Photoprocesses in Biopolymers 175 -- 6.5 In Vivo Photoexcitation 186 -- 6.6 In Vivo Spectroscopy 190 -- 6.7 Optical Biopsy 191 -- 6.8 Single-Molecule Detection 195 -- 7. Bioimaging: Principles and Techniques 203 -- 7.1 Bioimaging: An Important Biomedical Tool 205 -- 7.2 An Overview of Optical Imaging 206 -- 7.3 Transmission Microscopy 209 -- 7.4 Fluorescence Microscopy 219 -- 7.5 Scanning Microscopy 220 -- 7.6 Inverted and Upright Microscopes 221 -- 7.7 Confocal Microscopy 221 -- 7.8 Multiphoton Microscopy 223 -- 7.9 Optical Coherence Tomography 225 -- 7.10 Total Internal Reflection Fluorescence Microscopy 228 -- 7.11 Near-Field Optical Microscopy 232 -- 7.12 Spectral and Time-Resolved Imaging 234 -- 7.13 Fluorescence Resonance Energy Transfer (FRET) Imaging 237 -- 7.14 Fluorescence Lifetime Imaging Microscopy (FLIM) 238 -- 7.15 Nonlinear Optical Imaging 240 -- 7.16 Future Directions of Optical Bioimaging 245 -- 7.17 Some Commercial Sources of Imaging Instruments 246 -- 8. Bioimaging: Applications 255 -- 8.1 Fluorophores as Bioimaging Probes 256 -- 8.2 Green Fluorescent Protein 269 -- 8.3 Imaging of Organelles 271 -- 8.4 Imaging of Microbes 273 -- 8.5 Cellular Imaging 276 -- 8.6 Tissue Imaging 289 -- 8.7 In Vivo Imaging 294 -- 8.8 Future Directions 301 -- 8.9 Commercially Available Optical Imaging Accessories 303 -- 9. Optical Biosensors 311 -- 9.2 Principles of Optical Biosensing 314 -- 9.3 Support for and Immobilization of Biorecognition Elements 323 -- 9.4 Fiber-Optic Biosensors 327 -- 9.5 Planar Waveguide Biosensors 331 -- 9.6 Evanescent Wave Biosensors 334 -- 9.7 Interferometric Biosensors 338 -- 9.8 Surface Plasmon Resonance Biosensors 339 -- 9.9 Some Recent Novel Sensing Methods 343 -- 9.10 Future Directions 347 -- 9.11 Commercially Available Biosensors 349 -- 10. Microarray Technology for Genomics and Proteomics 357 -- 10.1 Microarrays, Tools for Rapid Multiplex Analysis 358 -- 10.2 DNA Microarray Technology 363 -- 10.3 Protein Microarray Technology 368 -- 10.4 Cell Microarray Technology 375 -- 10.5 Tissue Microarray Technology 379 -- 10.6 Some Examples of Application of Microarrays 379 -- 10.7 Future Directions 382 -- 10.8 Companies Producing Microarrays 383 -- 11. Flow Cytometry 390 -- 11.1 A Clinical, Biodetection, and Research Tool 391 -- 11.2 Basics of Flow Cytometry 394 -- 11.3 Fluorochromes for Flow Cytometry 405 -- 11.4 Data Manipulation and Presentation 408 -- 11.7 Commercial Flow Cytometry 426 -- 12. Light-Activated Therapy: Photodynamic Therapy 433 -- 12.1 Photodynamic Therapy: Basic Principles 434 -- 12.2 Photosensitizers for Photodynamic Therapy 437 -- 12.3 Applications of Photodynamic Therapy 447 -- 12.4 Mechanism of Photodynamic Action 450 -- 12.5 Light Irradiation for Photodynamic Therapy 453 -- 12.6 Two-Photon Photodynamic Therapy 455 -- 12.7 Current Research and Future Directions 457 -- 13.1 Tissue Engineering and Light Activation 465 -- 13.2 Laser Tissue Contouring and Restructuring 467 -- 13.3 Laser Tissue Welding 472 -- 13.4 Laser Tissue Regeneration 475 -- 13.5 Femtolaser Surgery 476 -- 14. Laser Tweezers and Laser Scissors 482 -- 14.1 New Biological Tools for Micromanipulation by Light 483 -- 14.2 Principle of Laser Tweezer Action 487 -- 14.3 Design of a Laser Tweezer 490 -- 14.4 Optical Trapping Using Non-Gaussian Beams 495 -- 14.5 Dynamic Holographic Optical Tweezers 496 -- 14.6 Laser Scissors 499 -- 14.7 Selected Examples of Applications 502 -- 14.9 Commercially Available Laser Microtools 514 -- 15. Nanotechnology for Biophotonics: Bionanophotonics 520 -- 15.1 Interface of Bioscience, Nanotechnology, and Photonics 521 -- 15.2 Nanochemistry 523 -- 15.3 Semiconductor Quantum Dots for Bioimaging 528 -- 15.4 Metallic Nanoparticles and Nanorods for Biosensing 532 -- 15.5 Up-Converting Nanophores 532 -- 15.6 PEBBLE Nanosensors for In Vitro Bioanalysis 536 -- 15.7 Nanoclinics for Optical Diagnostics and Targeted Therapy 537 -- 16. Biomaterials for Photonics 545 -- 16.1 Photonics and Biomaterials 545 -- 16.2 Bioderived Materials 548 -- 16.3 Bioinspired Materials 559 -- 16.4 Biotemplates 560 -- 16.5 Bacteria as Biosynthesizers for Photonic Polymers 564 -- 16.6 Future Directions 567.
Summary Paras Prasad's text provides a basic knowledge of a broad range of topics so that individuals in all disciplines can rapidly acquire the minimal necessary background for research and development in biophotonics. Introduction to Biophotonics serves as both a textbook for education and training as well as a reference book that aids research and development of those areas integrating light, photonics, and biological systems. Each chapter contains a topic introduction, a review of key data, and description of future directions for technical innovation. Introduction to Biophotonics covers the basic principles of Optics Optical spectroscopy Microscopy
Each section also includes illustrated examples and review questions to test and advance the reader's knowledge. Sections on biosensors and chemosensors, important tools for combating biological and chemical terrorism, will be of particular interest to professionals in toxicology and other environmental disciplines. Introduction to Biophotonics proves a valuable reference for graduate students and researchers in engineering, chemistry, and the life sciences.
Subject Photobiology.
Photonics.
Biosensors.
Nanotechnology.
ISBN 0471287709 (cloth)