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Title Quantitative real-time PCR : methods and protocols / edited by Roberto Biassoni, Alessandro Raso.

Published New York, NY : Humana Press, 2014.


Location Call No. Status
Physical description 1 online resource (xi, 231 pages) : illustrations (some color).
Series Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1160
Methods in molecular biology (Clifton, N.J.) ; 1160. 1064-3745
Notes Includes index.
Summary Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ? Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation.
Notes Description based on online resource; title from PDF title page (SpringerLink, viewed April 25, 2014).
Other author Biassoni, Roberto, editor.
Raso, Alessandro, editor.
Subject Polymerase chain reaction -- Laboratory manuals.
Electronic books.
ISBN 9781493907335 (electronic bk.)
1493907336 (electronic bk.)
Standard Number 10.1007/978-1-4939-0733-5